The molecular phylogenetic analysis of selected Galanthus species from Northwest Turkey

Abstract

ÖZETMarmara bölgesinde bulunan üç Galanthus (Kardelen) türünün moleküler filogenetikGalanthus plicatus subsp. byzantinus, Galanthus nivalis veLQFHOHPHOHUL /DSÃOGÃmorfolojik olarak hibritleri olarak kabul edilen Galanthus xvalentinei nothosubsp.subplicatus'un çekirdekte bulunan ribosomal DNA (ITS) bölgesi ile kloroplastintronun ve trnL(UAA)-trn)*$$LQWURQODUÃQÃQ trnL(UAA) JHQOHUL DUDVÃQGDNLnivalis GL/HWDQÃPODQDQELWNLOHUEOJHQLQVHNDQVODUÃDQDOL]HGLOGL0RUIRORMLNRODUDNG.G. nivalisµGHQ ve G. xvalentineiJHQHWLN DoÃGDQ $YUXSD¶GD NDEXO HGLOHQ IDUNOÃnothosubsp. subplicatusUQHNOHULLOHD/QÃROGX÷XDQODúÃOGÃ. G. xvalentinei nothosubsp.subplicatus UQHNOHUL LVH KLoELU PROHNÂOHU EHOLUWHoWH KLEULWOHúPH EHOLUWLOHUL YH/DGH÷LúNHQOLNJVWHUPHGL)LORJHQHWLNDQDOL]OHJHQHWLN RODUDND/Uà YH VDELWELUWÂUROGX÷XG. plicatus subsp. byzantinusDQODúÃOGà 0RUIRORMLN RODUDN RODUDN WDQÃPODQDQ ELWNLOHUnükleer DNA seviyesinde G. plicatus¶D /DNÃQ GXUGX $PD NORURSODVW VHNDQVODUà G.xvalentinei nothosubsp. subplicatus¶D oRN EHQ]HU oÃNWà %X LNL JHQRP DUDVÃQGDNLG.X/XPVX]OXN HVNL ELU KLEULW RODELOHFH÷L DQODPÃQD JHOHELOLU %X VRQXoODU 7ÂUNL/H¶GHnivalis G. xvalentinei nothosubsp.EXOXQPDGÃ÷ÃQD GDLU NDQÃWODU VXQPDNWD YHsubplicatus¶XQKLEULWGH÷LOD/UÃELUWDNVRQRPLNÂQLWHKDOLQGH/HQLGHQDGODQGÃUÃOPDVÃQÃYH SRSÂODV/RQ LQFHOHPHOHUL/OH ELUOLNWH /HQL ELU NRUXPD VWUDWHMLVLQLQ ROXúWXUXOPDVÃQÃgerektirmektedir.

ABSTRACTThe molecular phylogenetic relationship of the Galanthus taxa found in Northwest Turkeywas investigated. Galanthus plicatus subsp. byzantinus, Galanthus nivalis and theirpotential hybrid Galanthus xvalentinei nothosubsp. subplicatus were analyzed usingribosomal DNA (ITS) region and chloroplast introns (trnL(UAA) intron, intragenic spacerbetween trnL(UAA)-trnF(GAA)). The samples morphologically identified as G. nivalisand found in Turkey, did not genetically match G. nivalis recognized in Europe, they wereidentical to the samples defined as G. xvalentinei nothosubsp. subplicatus. G. xvalentineinothosubsp. subplicatus samples showed no indications of recent hybridization orinstability in any of the molecular markers examined. They were placed on a separate nodeindicating a genetically stable taxonomic unit, showing a range of morphological markers.The samples morphologically identified as G. plicatus subsp. byzantinus were on thenuclear DNA level very similar to G. plicatus. The chloroplast sequences, however, werevery similar to those of G. xvalentinei nothosubsp. subplicatus. This could be indicative ofan ancient hybrid but currently a stable species. These results show that the presence of G.nivalis in Turkey seems highly unlikely, and that G. xvalentinei nothosubsp. subplicatusshould be defined as a separate stable taxonomic unit that requires recognition and furtherpopulation ecology research.