Deneysel periodontal defektlerde uygulanan kolojen membranların yönlendirilmiş doku rejenerasyonu tekniği içerisindeki etkinlikleri ve rezobsiyon özelliklerinin incelenmesi

Abstract

94 SUMMARY It has been determined that the ideal cell type is the periodontal ligament cell population in order to obtain the regeneration on teeth which have lost its supporting structure as a result of periodontal diseases. Guided tissue regeneration technique has been extensively used in order to help the repopulation of these cells in the defective areas. With this technique which has been applied by using the resorbable and non-resorbable membranes, the proliferation of periodontal ligament cells into the coronal direction has been allowed while the epithelium and gingival connective tissue has been excluded from the healing area. Present study has been planned to assess the capacity of a resorbable collagen membrane to prevent the apical migration of epithelium and produce new attachment. In addition, the resorption characteristics of the membrane have been investigated as well. For this purpose, collagen membranes rehydrated with fibronectin solution were placed over some of the experimental defects and membranes rehydrated with saline only were placed over the rest of the defects which were created on the mandibular the 2nd, 3rd and 4tn premolar teeth of 6 dogs. The 3rd premolar teeth were used as control. On the control defects only root planning and flap operation were performed. Animals were sacrificed on the 7th, 28tn and 60tn days following the surgical procedures and block biopsies were obtained. Gingival tissue samples were taken from all areas of study for the electron microscopic evaluation on the 7*h95 day. Histopathologic and histometric analysis has revealed that both of the membrane groups and the control group have similar effects to prevent the downgrowth of epithelium. More new attachment has been observed in the membrane groups but no statistical difference between the membrane groups and the control group has been found(P>0.05). Likewise, new bone formation has proved to be more in membrane groups but there has been found no statistical difference on the 28th day(P>0.05). Only biometric differences have been found between the membrane groups enriched with fibronectin and the control group on the 60^ day(P«0.05). It has been found that the collagen membranes are tolerated well with the tissues and their resorption begin in the coronal area within the first 7 days. With the electron microscopic examination done on the 7*h day it has been concluded that the membranes enriched with fibronectin solution have resorbed more slowly at the ultrasutructural level. No membrane remnants have been identified on the 60th day.

94 SUMMARY It has been determined that the ideal cell type is the periodontal ligament cell population in order to obtain the regeneration on teeth which have lost its supporting structure as a result of periodontal diseases. Guided tissue regeneration technique has been extensively used in order to help the repopulation of these cells in the defective areas. With this technique which has been applied by using the resorbable and non-resorbable membranes, the proliferation of periodontal ligament cells into the coronal direction has been allowed while the epithelium and gingival connective tissue has been excluded from the healing area. Present study has been planned to assess the capacity of a resorbable collagen membrane to prevent the apical migration of epithelium and produce new attachment. In addition, the resorption characteristics of the membrane have been investigated as well. For this purpose, collagen membranes rehydrated with fibronectin solution were placed over some of the experimental defects and membranes rehydrated with saline only were placed over the rest of the defects which were created on the mandibular the 2nd, 3rd and 4tn premolar teeth of 6 dogs. The 3rd premolar teeth were used as control. On the control defects only root planning and flap operation were performed. Animals were sacrificed on the 7th, 28tn and 60tn days following the surgical procedures and block biopsies were obtained. Gingival tissue samples were taken from all areas of study for the electron microscopic evaluation on the 7*h95 day. Histopathologic and histometric analysis has revealed that both of the membrane groups and the control group have similar effects to prevent the downgrowth of epithelium. More new attachment has been observed in the membrane groups but no statistical difference between the membrane groups and the control group has been found(P>0.05). Likewise, new bone formation has proved to be more in membrane groups but there has been found no statistical difference on the 28th day(P>0.05). Only biometric differences have been found between the membrane groups enriched with fibronectin and the control group on the 60^ day(P«0.05). It has been found that the collagen membranes are tolerated well with the tissues and their resorption begin in the coronal area within the first 7 days. With the electron microscopic examination done on the 7*h day it has been concluded that the membranes enriched with fibronectin solution have resorbed more slowly at the ultrasutructural level. No membrane remnants have been identified on the 60th day.