Kan ve süt serumlarında enfeksiyöz bovine rhinotraccheitis-enfeksiyöz pustuler vulvovagenitis (IBR_IPV) antikorlarının nötralizasyon testi ile saptanması ve süt örneklerinden virüs izolasyonu

Abstract

88 7. ÎNGÎLÎZCE ÖZET (Summary) DETECTION OF ANTIBODIES OF IER/IPV INFECTION IN BLOOD AND MILK SERA BY SERUM NEUTRALISATION TEST AND VIRUS ISOLATION FROM MILK SAMPLES IN DAIRY COWS In this study, the presence of antibody BHV1 in the samples of blood and milk obtained from 486 cows that 390 of them were healty appearance or showed the symptoms of IBR-IPV infection and 96 were suffered from mastitis in 10 install type dairy farms located in various parts of Turkey were investigated by microneutralisation test. The role of BHV1 in the samples of milk obtained from 96 cows with mastitis in epidemiology of the disease was examined. The usage of milk serum in the diagnosis of IBR-IPV infection as an alternative to blood serum was controlled by the microneutralisation test. Virus isolation in the sensitive cell culture (FCK,MDBK) was studied by controlling the samples of milk obtained from 96 cows with mastitis for BHV1. At the end of the experiment, 359 blood sera samples (74 %) and 209 milk sera samples (43 %) were found to be positive for BHV1 antibody. The number of cows whose both of the samples of milk and blood were positive for BHV1 antibody was 209.89 The most reliable results were obtained from both of the samples than that of one in the diagnosis of serology of IBR-IPV. It was observed that milk samples were also suitable material like blood samples for the laboratory control of infection. Virus isolation from one sample of milk obtained from 96 cows with mastitis cows was made and identified as BHV1 according to SN and EM results. It was concluded that BHV1 was considered as a factor in mastitis condition and milk obtained from cows with mastitis could play an important role in the epidemiology of disease.

88 7. ÎNGÎLÎZCE ÖZET (Summary) DETECTION OF ANTIBODIES OF IER/IPV INFECTION IN BLOOD AND MILK SERA BY SERUM NEUTRALISATION TEST AND VIRUS ISOLATION FROM MILK SAMPLES IN DAIRY COWS In this study, the presence of antibody BHV1 in the samples of blood and milk obtained from 486 cows that 390 of them were healty appearance or showed the symptoms of IBR-IPV infection and 96 were suffered from mastitis in 10 install type dairy farms located in various parts of Turkey were investigated by microneutralisation test. The role of BHV1 in the samples of milk obtained from 96 cows with mastitis in epidemiology of the disease was examined. The usage of milk serum in the diagnosis of IBR-IPV infection as an alternative to blood serum was controlled by the microneutralisation test. Virus isolation in the sensitive cell culture (FCK,MDBK) was studied by controlling the samples of milk obtained from 96 cows with mastitis for BHV1. At the end of the experiment, 359 blood sera samples (74 %) and 209 milk sera samples (43 %) were found to be positive for BHV1 antibody. The number of cows whose both of the samples of milk and blood were positive for BHV1 antibody was 209.89 The most reliable results were obtained from both of the samples than that of one in the diagnosis of serology of IBR-IPV. It was observed that milk samples were also suitable material like blood samples for the laboratory control of infection. Virus isolation from one sample of milk obtained from 96 cows with mastitis cows was made and identified as BHV1 according to SN and EM results. It was concluded that BHV1 was considered as a factor in mastitis condition and milk obtained from cows with mastitis could play an important role in the epidemiology of disease.