Farklı irrigasyon solüsyonlarının periapikal dokularda oluşturduğu histopatolojik reaksiyonların incelenmesi

Abstract

70 dolgusunun raciyografik kontrolü yapıldıktan sonra giriş kaviteieri amaigam dolgu ile kapatıldı. Deney süresi olarak belirlenen 2, 30 ve 90 günlük dönemlerin sonunda hayvanlar dekapite edildiler. Deneylerin yapıldığı dişler çevre dokular ile birlikte blok kesitler halinde çıkartılıp rutin laboratuvar işlemleri ile histolojik kesitler hazırlandı. Hazırlanan preparatlar ışık mikroskobu altında histopatolojik yönden değerlendirildi. İncelenen kesitlerde 2 günlük deney süresinde kontrol grubu ve 1. deney grubunda hafif düzeyde, 2. deney grubunda ise orta düzeyde inflamasyon izlendi. 30 günlük deney süresinde kontrol grubu ve 1. deney grubuna ait örneklerde infiamasyona rastlanmazken, 2. deney grubunda hafif düzeyde inflamasyon tespit edildi. 90 güniük deney süresi sonunda ise her üç gruba ait örneklerin tümünde periapikai dokuların normal histolojik sınırlarda oiduğu gözlendi. Anahtar Keiimeier: Irrigasyon solüsyonları, sitrik asit, EDTA, NaOCI, histopatoiojik inceieme, periapikai doku.SUMMARY Histopathoiogical Evaluation of Periapical Tissue Reactions Against to Different irrigation Solutions Histopathoiogical changes of periapical tissues caused by various irrigation solutions that can remove smear layer during cnemomechanic preparation in the root canais; were examined under in vivo conditions. For this purpose, 48 mandibular premolar teeth of 6 mongrel dogs were used. Pulp tissues were extirpated by using barbed broaches of various sizes. After determining the working length with the aid of periapical radiographs, biomechanic preparation were carried out by using K-type files of 15-40 for the 1st premolar teeth and 20-45 for the 2`d, 3rd and 4,h premolar teeth. Three different experimental groups were formed to determine the effects of irrigation solutions during the cnemomechanic preparation. In the control group, conventional irrigation solutions which are 5% NaOCi and 3% H202 were used. 17% EDTA - 5% NaOCI in the 1s1 and 15% citric acid - 5% NaOCI 2Rd experimental groups were used for the irrigation. After application of the last size canal instrument, EDTA for the 1st group and citric acid for the 2no group were left in the canals for 60 seconds, and finally root canals were irrigated by distilled water. Following the cnemomechanic preparation, root canals were dried and filled with Seaiapex and gutta percha cones by lateral condensation technique. After radiographic controls of root canal fiilings, access cavities were restored with amalgam.

70 dolgusunun raciyografik kontrolü yapıldıktan sonra giriş kaviteieri amaigam dolgu ile kapatıldı. Deney süresi olarak belirlenen 2, 30 ve 90 günlük dönemlerin sonunda hayvanlar dekapite edildiler. Deneylerin yapıldığı dişler çevre dokular ile birlikte blok kesitler halinde çıkartılıp rutin laboratuvar işlemleri ile histolojik kesitler hazırlandı. Hazırlanan preparatlar ışık mikroskobu altında histopatolojik yönden değerlendirildi. İncelenen kesitlerde 2 günlük deney süresinde kontrol grubu ve 1. deney grubunda hafif düzeyde, 2. deney grubunda ise orta düzeyde inflamasyon izlendi. 30 günlük deney süresinde kontrol grubu ve 1. deney grubuna ait örneklerde infiamasyona rastlanmazken, 2. deney grubunda hafif düzeyde inflamasyon tespit edildi. 90 güniük deney süresi sonunda ise her üç gruba ait örneklerin tümünde periapikai dokuların normal histolojik sınırlarda oiduğu gözlendi. Anahtar Keiimeier: Irrigasyon solüsyonları, sitrik asit, EDTA, NaOCI, histopatoiojik inceieme, periapikai doku.SUMMARY Histopathoiogical Evaluation of Periapical Tissue Reactions Against to Different irrigation Solutions Histopathoiogical changes of periapical tissues caused by various irrigation solutions that can remove smear layer during cnemomechanic preparation in the root canais; were examined under in vivo conditions. For this purpose, 48 mandibular premolar teeth of 6 mongrel dogs were used. Pulp tissues were extirpated by using barbed broaches of various sizes. After determining the working length with the aid of periapical radiographs, biomechanic preparation were carried out by using K-type files of 15-40 for the 1st premolar teeth and 20-45 for the 2`d, 3rd and 4,h premolar teeth. Three different experimental groups were formed to determine the effects of irrigation solutions during the cnemomechanic preparation. In the control group, conventional irrigation solutions which are 5% NaOCi and 3% H202 were used. 17% EDTA - 5% NaOCI in the 1s1 and 15% citric acid - 5% NaOCI 2Rd experimental groups were used for the irrigation. After application of the last size canal instrument, EDTA for the 1st group and citric acid for the 2no group were left in the canals for 60 seconds, and finally root canals were irrigated by distilled water. Following the cnemomechanic preparation, root canals were dried and filled with Seaiapex and gutta percha cones by lateral condensation technique. After radiographic controls of root canal fiilings, access cavities were restored with amalgam.