Meme kanser hücrelerinde ErbB reseptör dağılımı ve hücre proliferasyonunun immünositokimyasal yönetme ile incelenmesi

Abstract

ÖZET%XoDOÃúPDGDPHPHNDQVHULKÂFUHVR/ODUÃ0'$0% YH7' KÂFUHOHULQGH(UE%UHVHSWUOHULQLQ GD÷ÃOÃPÃQà YH KÂFUHOHULQ SUROLIHUDV/RQ ]HOOLNOHULQL LPPXQRVLWRNLP/DVDOyöntemle inceledik. MDA MB 231 hücrelerinde ErbB1 (EGFR) ve ErbB2 reseptörlerinukleXV LoLQGH /R÷XQ RODUDN VDSWDQÃUNHQ 7' KÂFUHOHULQGH VH/UHN RODUDN KÂFUHPHPEUDQÃQGD ER/DQGÃNODUà JUÂOG (UE% UHVHSWUOHUL LVH KHU LNL KÂFUH VR/XQGD (UE%UHVHSWUOHULQH EHQ]HU IDNDW GDKD D] /R÷XQ ELU GD÷ÃOÃP J]OHGLN +ÂFUH LoL WLUR]LQfosforilasyonunX LQFHOHGL÷LPL]GH (UE% UHVHSWU GD÷ÃOÃPÃQD EHQ]HU ELU GD÷ÃOÃP JVWHUGL$/UÃFD KÂFUHOHULQ SUROLIHUDV/RQ GÂ]H/LQL 3&1$ HNVSUHV/RQX YH %UG8 LQNRUSRUDV/RQX LOHimmunositokimyaVDO/QWHPOHLQFHOHGL÷LPL]GH0'$0%KÂFUHOHULQLQ7'KÂFUHOHULQHJUHNDWGDKDKÃ]OÃSUROLIHUHROGXNODUÃQÃJUGÂNSonuç olarak, MDA MB 231 hücrelerinde ErbB reseptör ailesinin nükleerORNDOL]DV/RQ JVWHUPHVL YH SUROLIHUDV/RQ KÃ]ÃQÃQ GDKD /ÂNVHN ROPDVà EX KÂFUHOHULQ 7'KÂFUHOHULQH JUH GDKD PDOLJQ ELU NDUDNWHUGH ROGX÷XQX JVWHUPHNWHGLU $/UÃFD (UE% UHVHSWUHNVSUHV/RQXQD UD÷PHQ KÂFUH LoL IRVIRWLUR]LQ DNWLYLWHVLQLQ D] ROPDVà EX KÂFUHOHUGH EDúNDIDNWUOHULQGHKÂFUHoR÷DOPDVÃQDNDWNÃGDEXOXQGX÷unuGÂúÂQGÂUPHNWHGLU

SUMMARYIn the present study we have investigated the distribution of ErbB receptors and thecharacteristics of cell proliferation in breast cancer cell lines MDA MB 231 and T47D byimmunocytochemical analysis. In MDA MB 231 cells, ErbB1 (EGFR) and ErbB2 receptorswere stained in the nucleus, while in T47D cells they were detected in the cell membrane. Thedistribution of ErbB3 receptors was similar but less dense than the ErbB2 receptors in bothcell lines. The distribution of tyrosine phosphorylation was also found similar to ErbB3receptors. In addition, when we investigated the level of cellular proliferation with PCNAexpression and BrdU incorporation, we found that the rate of cell proliferation in MDA MB231 cells was 2.5 fold higher than T47D cells.In conclusion, the nuclear localization of ErbB family receptors in MDA MB 231 andtheir higher rate of proliferation indicate the malignant phenotype of these cells compared toT47D cells. In addition, in the presence of ErbB receptor expression, the low level of tyrosinephosphorylation suggests the involvement of other factors in the regulation of cellproliferation in these cells.