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dc.contributor.advisorYağcı, Tuba
dc.contributor.authorŞen, Eda
dc.date.accessioned2021-05-07T07:49:03Z
dc.date.available2021-05-07T07:49:03Z
dc.date.submitted2017
dc.date.issued2018-08-06
dc.identifier.urihttps://acikbilim.yok.gov.tr/handle/20.500.12812/595101
dc.description.abstractİç Anadolu bölgesinde yayılış gösteren Nannospalax xanthodon' un 2n = 54 ve 2n = 60 sitotiplerine ait toplam 36 örnek ISSR-PCR (Inter Simple Sequence Repeat-Polymerase Chain Reaction) tekniği kullanılarak analiz edildi. Optimizasyonu sağlanan 7 ISSR primerinden ((AG)8T, (GGAGA)5, (GACA)4, (TG)8A, (CAG)5GC, (CAG)4AC ve (GA)8AC) 101'i polimorfik olmak üzere 112 bant üretildi. Bu primerlerden (AG)8T ve (GA)8AC primerleri 2n = 54 ve 2n = 60 sitotiplerine özgü bantlar üreterek, sitotipleri ayırt edebilmek için en bilgilendirici oldu. En iyi optimizasyonun sağlandığı markörler aracılığıyla belirlenen polimorfizm oranları kullanılarak 'Genetik mesafe matriksi' hesaplandı. Jaccard katsayısına dayalı olarak hesaplanan UPGMA (Unweighted Pair Group Mean Average) analizinde iki sitotipin belirgin olarak ayrıldığı tespit edildi.
dc.description.abstractA total of 36 samples of 2n = 54 and 2n = 60 cytotypes of Nannospalax xanthodon, spreading in the Central Anatolia region, were analysed by using ISSR-PCR (Inter-Simple Sequence Repeat-Polymerase Chain Reaction) technique. 112 bands were produced, 101 of which were polymorphic, from optimised 7 ISSR primers ((AG)8T, (GGAGA)5, (GACA)4, (TG)8A, (CAG)5GC, (CAG)4AC and (GA)8AC). (AG)8T and (GA)8AC primers which were from these primers were most informative to distinguish cytotypes by producing bands specific for 2n = 54 and 2n = 60 cytotypes. The genetic distance matrix was calculated using the polymorphism rates determined by the markers provided with the best optimisation. It was determined that the two cytotypes were distinctly separated in the UPGMA (Unweighted Pair Group Mean Average) analysis calculated based on the Jaccard coefficient.en_US
dc.languageTurkish
dc.language.isotr
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution 4.0 United Statestr_TR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiyolojitr_TR
dc.subjectBiologyen_US
dc.subjectGenetiktr_TR
dc.subjectGeneticsen_US
dc.titleİç Anadolu bölgesinde yayılış gösteren Nannospalax xanthodon Satunin, 1898 (Mammalia:Rodentia)`un 2n = 54 ve 2n = 60 sitotiplerinin ISSR tekniği kullanılarak moleküler filogenisi
dc.title.alternativeMolecular phylogeny of the 2n=54 and 2n=60 cytotypes of Nannospalax xanthodon Satunin, 1898 (Mammalia:Rodentia) from Central Anatolia by ISSR marker technique
dc.typemasterThesis
dc.date.updated2018-08-06
dc.contributor.departmentMoleküler Biyoloji ve Genetik Ana Bilim Dalı
dc.identifier.yokid10160990
dc.publisher.instituteFen Bilimleri Enstitüsü
dc.publisher.universityBİLECİK ŞEYH EDEBALİ ÜNİVERSİTESİ
dc.identifier.thesisid472873
dc.description.pages50
dc.publisher.disciplineDiğer


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