Deney hayvanlarında ağrılı uyaranların davranış parametrelerine etkisi ve cinsiyetin önemi

Abstract

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THE EFFECTS OF STIMULANTS ON BEHAVIORAL PARAMETERS AND THEIMPORTANCE OF SEX ON EXPERIMENTAL ANIMALSABSTRACTIt this study, we investigated the affects of phasic and tonic pain in the form of hot plate,acetic acit, formalin test which on the behaviour of male and female rats and mice. Theirbehaviour was observed on open-field and elevated T-maze.Each pain stimulant was appliedto 3 groups of 20 experimental animals, 10 male and 10 female.A total of 60 experimentalanimals were used in the study, 20 wistar Albino rats and 40 Balb-c mice.In the hot plate tests, the animals were placed on the hot plate wich was heated at 52Û&,andthe time period up to the animals first licking or pulling up period up their paws was recorded.In the acetic acit test, after the animals were injected with 6%, 1.5 ml/kg i.p the writingmovement were counted. In the formalin application , 37%, 1.35ml/kg formalin was injecteds.c.into the rats feet/paws.Prior to and following the application of the pain stimulants, their behaviour parameters wereobserved in the elevated T-maze and open-field area. In the open-field the section theanimals crossed were counted and determined as their locomotor activity, the number of timestheir reared was counted their exploration behaviour and the number of groming anddefecation were counted as a value of their autonomic fuction.The base levels avoidance and escape times were investigated. In the elevated T- mazetest.The phasic pain response was not effected by the sex of the animals in the hot plate test.Inthe acetic acit test, it was found that female animals were more sensitive, although in phase1.(0-10 minutes) of the formalin applications on the males were more sensitive, although inphase2.(10-60 minutes) the females were more sensitive. In the open field test, followed theapplication of the 3 pain stimulants, their behaviour parameters decreased. This decrease wasmost significant in the acetic acit application . The elevated T-maze test behaviour parameterswere elongated following the 3 pain stimulants.From the results of this study, we observed that behaviour is effected by pain and sex in animportant factor in some pain testsKey words: pain, behaviour, open field, elevated T-maze, mice, rat.