Elektrodöndürme tekniğiyle oluşturulan polimer nano-liflerin memeli hücresi etkileşimlerinin incelenmesi

Abstract

In this study, scaffolds with various size and thickness were prepapared from a poly[(D,L-lactic-co-glycolic acid) (85:15)] (PLGA) copolymer with biocompatible and biodegradable properties by using the electrospinning technique. To achieve this objective, electrospinning parameters such as applied voltage, concentration of polymer solution, solvent/solvent systems, distance between needle and collecting plate, pump rate and finally needle inner diameter were optimized. Morphological properties of the formed nanotopographic membrane scaffolds such as nanofiber structure, nanofiber size, diameter range, etc. were evaluated by scanning electron microscopy (SEM) and mechanical properties were also determined by tensile tester. The effects of these scaffolds on human periodontal ligament fibroblastic cell adhesion, proliferation, differentiation and synthesis of extracellular matrix were evaluated under cell culture conditions. For this prupose the cell viability and mitochondrial dehidrogenase activity test (MTT), histology (H&E and Alizarin red) and immunohistochemistry (fibroblast surface protein, collagen type I, bone sialoprotein, osteopontin, and osteocalcin antibody stainings) assays were performed. 2007, 51 pages KeyWords: Electrospinning, PLGA, human periodontal ligament fibroblast cells, nanobiotechnology, periodontal tissue engineering, biomaterials, polymer nanofibers.