Deneysel kontrast nefropati modelinde melatoninin önleyici ve düzeltici etkisi

Abstract

dehydratation and furosemide was observed during the excreation of sodium in groups I (0,138±0,085 vs 0,057±0,042; p=0,043) and IV (0,160±0,097 vs 0,061±0,049; p=0,043), an increase was observed in group II (0,108±0,104 vs 0,202±0,262; p=0,893) and III (0,146±0,051 vs 0,373±0,477; p=0,917) due to the development the tubuler dysfunction. Contrast nephropathy could not be prevented when melatonin was applied to rats before the contrast medium, however it could be prevented when melatonin was applied before and after the contrast medium was observed. Key words: Melatonin, contrast nephropathy, protection 45

THE PROTECTIVE AND IMPROVEMENT EFFECTS OF MELATONIN IN THE EXPERIMENTAL MODEL OF CONTRAST NEPHROPATHY SUMMARY Four groups each consisting of six Sprague-Dawley type adult male rats were used to do research on the effects of melatonin in the protection and improvement of contrast nephropathy. In group I melatonin was applied before and after the contrast medium, in group II melatonin was applied only before the contrast medium. The group III was prepared as a control group which contrast medium given, group IV was prepared as a control group which contrast medium not given. Melatonin 10mg/kg was applied intraperitoneally and contrast medium (Iomeprol 10mg/kg) was applied intravenously. All of the rats were left waterless for 24 hours, than they were applied 10mg/kg furosemide intramuscularly and 10mg/kg indomethasin intraperitoneally to increase the frequency of contrast nephropathy. Basal and control levels of serum creatinin, creatinin clearance, urine volume, fractione sodium excreation, alkaline phosphatase, lactic dehydrogenase and gamma glutamyl- transferase were evaluated. There was, no significant difference between the levels of creatinin and creatinin clearance and also between the urine volume levels in group I and IV. In group II, a increase in serum creatinin, which does not reach a statistical significence level; a decrease in creatinin clearance; and an increase in the volume of urine were observed. An increase in serum creatinin (0,333±0,052 vs 2,33±3.02; p=0,042); a decrease in creatinin clearance (2,70±0,462 vs 1,28±1,02; p=0,046), an increase in the volume of urine (10,00±1,79 vs 15,67±6,06; p=0,046) were observed in group III. Although a decrease depending on 44dehydratation and furosemide was observed during the excreation of sodium in groups I (0,138±0,085 vs 0,057±0,042; p=0,043) and IV (0,160±0,097 vs 0,061±0,049; p=0,043), an increase was observed in group II (0,108±0,104 vs 0,202±0,262; p=0,893) and III (0,146±0,051 vs 0,373±0,477; p=0,917) due to the development the tubuler dysfunction. Contrast nephropathy could not be prevented when melatonin was applied to rats before the contrast medium, however it could be prevented when melatonin was applied before and after the contrast medium was observed. Key words: Melatonin, contrast nephropathy, protection 45