Behçet hastalığı üveitinde retinal-S antijeni ile çapraz reaksiyon veren bir HLA-B kökenli bir peptide (B27PD) T hücre yanıtı

Abstract

Immune response to retinal autoantigens may play a central role in the pathogenesis of uveitis. A synthetic peptide from a common sequence of various HLA-B molecules associated with uveitis such as HLA-B27 and 51 (B27PD), which shares aminoacid homologies with a retinal -S antigen derived peptide (PDS) was shown to be immunogenic in human and experimental uveitis. In this study, we investigated T cell responses to these 2 synthetic peptides inBehçet's disease patients with posterior uveitis (BD-PU,n=33) and compared with non-Behçet anterior uveitis (AU, n=14), HLA-B51+BP patients without uveitis (BD, n=15) and healthy controls (HC, n=32) in a 6-day proliferation assay. BD-PU patients had higher responses (stimulation index, Sl:2.8±1.2) than AU (Sl:1.5±0.4) and HC (Sl:1.1± 0.6) for BPD (p<0.0001). Responses PDS were also higher in BD patients (Sl:3.3±1.7) than AU (Sl:1.5±0.4) and HC (1.1±0.6) (p<0.0001). A significant correlation between the responses to PDS and B27PD was also observed (r=0.6, p<0.001). These results suggest that cellular immunity to cross-reactive Rets-S Ag and HLA-B derived peptides might play a role in the pathogenesis of posterior uveitis in BD Key Words: Behcet's disease, HLA-B51- derived peptide, cellular immunity