Analyses of promoters in Arabidopsis thaliana of 3 beta HSD and 5 beta POR genes isolated from digitalis species
| dc.contributor.advisor | Gürel, Ekrem | |
| dc.contributor.author | Aslam, Noreen | |
| dc.date.accessioned | 2021-05-07T08:32:43Z | |
| dc.date.available | 2021-05-07T08:32:43Z | |
| dc.date.submitted | 2018 | |
| dc.date.issued | 2018-10-10 | |
| dc.identifier.uri | https://acikbilim.yok.gov.tr/handle/20.500.12812/597227 | |
| dc.description.abstract | Yüksükotu olarak bilinen Digitalis ferruginea, kardiyak glikozitlerin üretimi için kullanılmaktadır. Progesteron 5β-redüktaz (5βPOR1 ve 5βPOR2) ve 3β-hidroksisteroid dehidrojenaz (3β-HSD) Digitalis türlerinde kardenolidin biyosentez yolundaki önemli anahtar basamak genleridir. Bu çalışmada 3β-HSD, 5βPOR1 ve 5β POR2 promotörleri Digitalis ferruginea'nın genomik DNA'sından İlk kez izole edilip, dizi analizi yapılmıştır. Promoterlerin sekans bilgileri, bu promoterlerin ışık tepkileriyle ilgili bol cis-elementlere sahip olduğunu ortaya çıkarmıştır. 5βPOR1promoter spesifik cis elemanları sirkadiyen ve ısı şokudur. 5βPOR2promoter spesifik düzenleyici elemanlar savunma, stres ve metil jasmonattır. Yara yanıt elementi, 3β-HSDpromoter'da spesifik olarak bulunmuştur. Çiçek dalma transformasyonu ile transform olmuş Arabidopsis bitkilerinde 3β-HSDpromoter | |
| dc.description.abstract | Digitalis ferruginea, known as foxglove, is famous for the production of secondary metabolites; i.e., cardiac glycosides. The progesterone 5β-reductase (5βPOR1 and 5βPOR2) and 3β-hydroxysteroid dehydrogenase (3β-HSD) are important key step genes in the pathway of biosynthesis of cardenolide in Digitalis species. For the first time, promoters of 3β-HSD, 5βPOR1 and 5β POR2 were isolated from genomic DNA of Digitalis ferruginea and sequence analysis was performed. Sequence information of the promoters revealed that these promoters have abundant cis-elements related to light responses. 5βPOR1promoter specific cis-elements are circadian and heat shock. 5βPOR2promoter specific regulatory elements are defense, stress and methyl jasmonate. Wound response element was found specifically in the 3β-HSDpromoter. Functional characterization of the 3β-HSDpromoter | en_US |
| dc.language | English | |
| dc.language.iso | en | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | Attribution 4.0 United States | tr_TR |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Biyoteknoloji | tr_TR |
| dc.subject | Biotechnology | en_US |
| dc.subject | Botanik | tr_TR |
| dc.subject | Botany | en_US |
| dc.subject | Genetik | tr_TR |
| dc.subject | Genetics | en_US |
| dc.title | Analyses of promoters in Arabidopsis thaliana of 3 beta HSD and 5 beta POR genes isolated from digitalis species | |
| dc.title.alternative | Digitalis türlerinden izole edilecek 3 beta HSD ve 5 beta POR genlerine ait promotorların Arabidopsis thaliana'da analizi | |
| dc.type | masterThesis | |
| dc.date.updated | 2018-10-10 | |
| dc.contributor.department | Biyoloji Ana Bilim Dalı | |
| dc.identifier.yokid | 10197773 | |
| dc.publisher.institute | Fen Bilimleri Enstitüsü | |
| dc.publisher.university | BOLU ABANT İZZET BAYSAL ÜNİVERSİTESİ | |
| dc.identifier.thesisid | 511544 | |
| dc.description.pages | 97 | |
| dc.publisher.discipline | Botanik Bilim Dalı |
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