Niklozamidin Gallerıa mellonella L. (Lepıdoptera: Pyralıdae)`nın bazı biyolojik ve fizyolojik özelliklerine etkisi

Abstract

Salisilanilidler (or. Niklozamidler) barsak ~eridi gibi parazitlerin mitokondrisinde oksidatiffosforilasyonu inhibe ederek etkisini gosterirler ve bu antiparazitik ila<;lar tIpta veveterinerlikte kullamlmaktadlr. Niklozamidin potansiyel antihelmintik aktivitesi memelilerdeiyi bir ~ekilde ortaya konulmu~, bu <;ah~mada da Oalleria mellonella larvalan kullamlarakniklozamidin in vivo insektisit etkisi ara~tlfllml~tIr. Niklozamid, 7. eyre larvalan, pup, erginevrelerinin ya~ama oramm onemli derecede dli~lirlirken, en yliksek konsantrasyonu (% 1,0)erg in geli~me sliresini onemli derecede uzatml~tIr. % 0,1 'lik niklozamid ile beslenildiginde,di~ilerin yumurta verimi ylikselmi~tir; bunun yamslra en yliksek konsantrasyonda (% 1,0) hi<;yumurta elde edilememi~tir. Niklozamidin en yliksek konsantrasyonunda (% 1,0) erkek erg inomlir uzunlugu artml~tIr. Aynca, niklozamid yumurta a<;lhmml da denenen tUmkonsantrasyonlarda onemli oranda dli~lirmli~tUr. % 0,1 'lik niklozamid konsantrasyonundamalondialdehit (MDA) miktan 4 kat, glutatyon-S-transferaz enzimi (OST) aktivitesi ise 2 katartml~tIr. Kontrol besinine gore (133,24 ± 23,6 nmol/mg protein) niklozamidin denenen111Ozet (devam ediyor)konsantrasyonlan protein karbonil (peG) miktanm en az 5 kat (701,24 - 808,02 nmol/mgprotein) onemli derecede arttlrml~tIr. Bu <;ah~mada Galleria mellonella model bocek olarakkullamlarak, boceklerle mlicadelede belirli klinik oneme sahip antihelmintik ila<;lann aktif~ekilde kullamlabilirligi belirtilmi~tir. Aynca, bu <;ah~manm sonu<;lan niklozamidinprooksidan etkisine bagh olarak biyolojik ve aym zamanda bocegin antioksidan savunmacevabl lizerine negatif etkisi oldugunu gostermi~tir.Anahtar Kelimeler: Galleria mellonella, niklozamid, ya~ama oram, malondialdehid, proteinkarbonil, Glutatyon S-transferaz, beslenmeBHim Kodu: 40l.02.0l

The salisilanilides (e.g. niclosamide) function by inhibiting mitocondrial oxidativephosphorylation in parasitic tapeworms and thus they are used as an antiparasitic drug inmedicine and veterinary. While the potent antihelmintic activity of niclosamide has been wellcharacterised in mammals, this study investigated the in vivo insecticide effect of niclosamideusing larvae of the insect Galleria mellonella. Niclosamide was successful in decreasing thesurvival of 7th instar larvae, pupal and adult stages while only the highest concentration ofthis antihelmintic antibiotic (1.0 %) significantly prolonged developmental time to adultstage. Females also increased their fecundity when reared with 0.1 % of niclosamide. On theother hand, we could not obtain any egg in (1.0 %) concentration. An increase in the maleadult longevity was obtained when reared with the highest concentrations of niclosamide.Niclosamid rearing resulted in an decrease in hatchability of eggs. Niclosamide at 0.1 % ofconcentration increased MDA content (4-fold), GST activity (2-fold). Relative to control(133.24 ± 23.6 nmol/mg protein), niclosamide at tested concentrations significantly increasedvABSTRACT (continued)pea content at least 5-fold (70l.24- 808.02 nmol/mg protein). This work indicates that G.mellonella larvae may be used as a good model to ascertain importance of clincally importantantihelmintic drug active ingredients in chemical management of pest insects. The results ofthis work also indicate the negative effects of niclosamide on insect biology is due to its prooxidantproperties and also to the ability of niclosamide in crippling the insect's antioxidandefence response.Key Words: Galleria mellonella, niclosamide, survivorship, malondialdehyde, proteincarbonyl, Glutathione S-transferase, nutritionScience Code: 40l.02.0l