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dc.contributor.advisorŞimşek, Halis
dc.contributor.authorAsil, Mehmet
dc.date.accessioned2020-12-29T13:50:24Z
dc.date.available2020-12-29T13:50:24Z
dc.date.submitted2000
dc.date.issued2018-08-06
dc.identifier.urihttps://acikbilim.yok.gov.tr/handle/20.500.12812/434210
dc.description.abstractHBV'ye bağlı hepatoselüler hasar oluşumunda immünolojik mekanizmaların anahtar rol oynadığı düşünülmektedir. İnterlökin-1 (IL-1), proinflamatuar sitokinlerin prototipidir T hücre aktivasyonunda görev alır. Bu çalışmada kronik hepatit B patogenezinde IL-lα'nın rolünün araştırılması planlandı. 25 kronik hepatit B hastası (grup 1) çalışmaya dahil edildi. 12 sağlıklı hepatit B taşıyıcısı (grup 2) ve 10 sağlıklı birey (grup 3) kontrol grubu olarak alındı. Grup 1'deki hastaların 21'i erkek, 4'ü kadın ve ortalama yaşları 31,4±8,0 yıl idi. Grup 2'de yaş ortalamaları 29,5±7,1 yıl olan 8 erkek ve 4 kadın ve grup 3'te yaş ortalamaları 28,4±4,3 yıl olan 7 erkek ve 3 kadın bulunuyordu. Grup l'deki hastaların karaciğer biyopsilerinin histolojik incelenmesinde inflamasyon, 3 hastada grade 1, 16 hastada grade 2 ve 6 hastada grade 3, fıbrosis ise 10 hastada stage 1, 9 hastada stage 2 ve 6 hastada stage 3 bulundu. Ortalama serum IL-lα konsantrasyonları grup 1,2 ve 3 için sırasıyla 4,20 ±0,87 pg/ml, 2,14±1,46 pg/ml ve 1,35±0,40 pg/ml olarak bulundu. Gruplar arasındaki fark istatistiksel olarak anlamlıydı (p<0,05). ALT, HBV DNA düzeyi ve HBe antijen durumu ile serum IL-lα konsantrasyonları arasında ilişki bulunamadı. Grade 1,2 ve 3 inflamasyonu olan hastalarda ortalama serum IL-lα konsantrasyonları sırasıyla 7,51±4,42 pg/ ml, 4,06±1,06 pg/ ml ve 2,92±0,96 pg/ ml ve stage 1,2,3 fıbrozisli hastalarda sırasıyla 3,59±1,45 pg/ml, 3,03±1,11 pg/ml ve 6,98±1,99 pg/ml idi ancak aradaki fark istatistiksel olarak anlamlı değildi. Sonuç olarak, kronik hepatit B'de serum IL-lα düzeyleri sağlıklı bireyler ve HBs taşıyıcılarına göre yüksektir. IL-lα kronik hepatit B 'ye bağlı hepatoselüler hasar ve fibrozis gelişiminde çeşitli mekanizmalarla rol oynayabilir, bu konunun daha detaylı aydınlatılabilmesı için geniş hasta serilerini içeren ileri çalışmalara ihtiyaç vardır.
dc.description.abstractThe complex pathophysiological mechanisms underlying viral persistence is not fully understood in chronic hepatitis B infection but immunologic mechanisms are believed to play a key role in HBV associated hepatocellular injury. Interleukine-1 (IL-1) is the prototype of proinflammatory cytokines. It is primarily secreted from mononuclear cells in response to various stimuli and involved in regulation of acute phase responses and T cell activation. This study is conducted to investigate the role of IL-lα in the pathogenesis of chronic hepatitis B infection. 25 patients with chronic HBV hepatitis were taken into the study. 10 healthy subjects and 12 healthy HBV carriers were selected as control groups. The chronic hepatitis B group (Group 1) consisted of 21 Males and 4 Females subjects with a mean age of 31.4±8.0 years. Healthy HBV carriers with normal aminotransferases (Group 2) consisted of 8 Males and 4 Females (mean age 29.5±7.1) and healthy controls (Group 3) consisted of 7 Males, 3 Females (mean age 28.4±4.3). Liver biopsy findings revealed, grade 1 necroinflammatory activity in 3, grade 2 in 16 and and grade 3 in 6 patients and stage 1 fibrosis in 10, stage 2 fibrosis in 9 and stage 3 fibrosis in 6 subjects. Serum samples were collected and serum IL-lα concentrations were determined with ELISA technique (R&D Systems, Oxon, UK). Mean serum IL- la concentration was higher in Group 1 (4.20 ±0.87 pg/ml) than the control groups (2.14±1.46 pg/ml in Group 2 and 1.35±0.40 pg/ml in Group 3. The difference was statistically significant (p<0.05). The difference between groups 2 and 3 was not significant (p>0.05). There was no correlation between ALT, HBV DNA levels or HBe antigen status and serum IL-la levels. There seemed to be a direct relation between fibrosis scores and an inverse relation between inflammation scores and serum IL-lα levels. Mean serum IL-lα levels were 7.51±4.42 pg/ml, 4.06±1.06 pg/ml, 2.92±0.96 pg/ml for grade 1,2 and 3 and 3.59±1.45 pg/ml, 3.03±1.11 pg/ml and 6.98±1.99 pg/ml for stage 1,2 and 3 fibrosis respectively. These differences were not statistically significant (p>0.05). In conclusion, serum IL-lα levels are elevated in chronic hepatitis B but no correlation between IL-lα levels and the level of inflammation and fibrosis in liver biopsies was found. Serum IL-lα may play role in the pathogenesis of chronic hepatitis B infection by means of disturbed immunologic and inflammatory mechanisms. Further studies with larger series are necessary to understand the exact role of IL-lα in the pathogenesis of the disease.en_US
dc.languageTurkish
dc.language.isotr
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution 4.0 United Statestr_TR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGastroenterolojitr_TR
dc.subjectGastroenterologyen_US
dc.titleKronik hepatit B hastalarında serum interleukin-1 α düzeyleri
dc.title.alternativeSerum interleukin-1 α levels in patients with chronic hepatitis B infection.
dc.typedoctoralThesis
dc.date.updated2018-08-06
dc.contributor.departmentİç Hastalıkları Anabilim Dalı
dc.identifier.yokid10145426
dc.publisher.instituteTıp Fakültesi
dc.publisher.universityHACETTEPE ÜNİVERSİTESİ
dc.type.submedicineThesis
dc.identifier.thesisid451227
dc.description.pages74
dc.publisher.disciplineGastroenteroloji Bilim Dalı


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