Gıdai indigasyonlu sığırlarda tiamin yetersizliğinin araştırılması

Abstract

62 ÖZET Bu çalışma, sığırların bazı gıdai indigesyonlarında, tiamin yetersizliğinin hangi düzeyde olduğunu belirlemek amacıyla yapılmıştır. Araştırmada, 20 baş sağlıklı (Kontrol) ve 30 baş gıdai indigesyonlu (10 baş rumen mikroflora ve faunasında hipoaktiviteli = HA; 20 baş rumen asidozisli = RA) inek kullanılmıştır. Gıdai indigesyon tanısı anamnez, klinik muayene bulguları ve rumen içeriği muayene bulgularına dayanılarak konulmuştur. Araştırmada kullanılan tüm hayvanların sistematik klinik muayeneleri yapılmıştır. Rumen içeriği muayeneleri (Koku, renk, kıvam, sedimentasyon, flotasyon, pH değeri, infusorya yoğunluğu, infusorya canlılık oranı ve metilen mavisi testi) ve biyokimyasal muayeneler ( Boehringer teşhis kitleriyle,total protein, L-Laktat, piruvat konsantrasyonları; O-Toluidin yöntemi ile glikoz konsantrasyonları; Clausen'in yöntemi ile de transketolaz enzim akti vitesi ve TPP etkisi değeri belirlenmiştir.) olmak üzere iki kısımda yapılan laboratuvar muayeneleri için, araştırma hayvanlarından alınan rumen içeriği ve kan örnekleri kullanılmıştır. Sağlıklı hayvanlarda ortalama vücut sıcaklığı, nabız ve solunum frekansı ile rumen hareketleri sayısı sırasıyla 38.4 °C, 77.1/dk, 24.4/dk ve 9.3/5 dk; rumen flora ve faunasında hipoaktiviteli hastalarda 38.7 °C, 77/dk, 25.2/dk ve 4 /5 dk; rumen asidozisli hastalarda ise 38.3 °C, 93.5/dk, 24.8//dk ve 0.4/5 dk olarak bulunmuştur. Vücut sıcaklığı ve solunum frekanslarında gruplar arasında önemli bir farklılık görülmemesine rağmen, nabız frekansında rumen asidozisli hayvanlarda, diğer gruplara göre önemli (p<0.001) derecede artış görülmüştür. Rumen hareketleri sayısı ise her iki grup hasta67 Total protein, glucose, pyruvate, L-Lactate and TPP effect values in healty animals were found to be 7.25 g/dl, 55.05 mg/dl, 0.17 mg/dl, 10.82 mg/dl and 18.95% respectively, whereas in cows with hypoactivity in rumen microflora and fauna, these values were 6.74 g/dl, 52.5 mg/dl, 0.26 mg/dl, 11.9 mg/dl and 38.2%; and in cows with rumen acidosis were 8.04 g/dl, 70.8 mg/dl, 0.4 mg/dl, 19.5 mg/dl and 46.65% respectively. Total protein value in cows with hypoactivity in rumen microflora and fauna was decreased (p<0.001) whereas in cows with rumen acidosis increased (p<0.001) compared to healty animals. Glucose and L-Lactate values in cows with rumen acidosis were increased significantly (p<0.001) compared to both healty cows and cows with hypoactivity in rumen microflora and fauna. Pyruvate value in both cows with hypoactivity in rumen microflora and fauna and with rumen acidosis were increased significantly (p<0.001) compared to healty animals. In addition pyruvate value in cows with rumen acidosis were increased significantly (p<0.001) compared to cows with hypoactivity in rumen microflora and fauna. Values of TPP effect in both cows with rumen acidosis and with hypoactivity in microflora and fauna were found to be increased significantly (p<0.001) compared to control group. In conclusion, as it can be seen from pyruvate, L-Lactate and especially TPP effect values, slight thiamin deficiency in cows with hypoactivity in rumen microflora and fauna and prominent thiamin deficiency in cows with rumen acidosis are present.Therefore, we believe that the administration of thiamin preparates especially in cows with rumen acidosis would be useful.66 pulse rate in cows with rumen acidosis were significantly greater (p<0.001) than the other groups. Number of rumen contractions in two groups of sick animals was decreased compared to healty animals (p<0.001). Furthermore number of rumen motility in cows with rumen acidosis were found to be decreased significantly (p<0.001) as compared to that in cows with hypoactivity in rumen microflora and fauna. Avarage sedimentation rate in cows with hypoactivity in rumen microflora and fauna was decreased compared to that healty animals, whereas the sedimentation rate in cows with rumen acidosis was increased. Avarage flotation rate was found to be increased in cows with hypoactivity in rumen microflora and fauna compared to healty animals whereas in cows with rumen acidosis, no flotation was detected. Mean pH value was increased in cows with hypoactivity in rumen microflora and fauna whereas decreased in cows with rumen acidosis as compared to healty animals. Intensity of infusoria was found to be (+++) in healty animals whereas in cows with hypoactivity in rumen microflora and fauna was decreased (+) and in cows with rumen acidosis no infusoria was detectable (-) with the exception of three animals. Rate of living infusoria was decreased in cows with hypoactivity in microflora and fauna, whereas in cows with rumen acidosis, it was found that all the infusoria were dead. Period of methylen blue test in cows with hypoactivity in microflora and fauna was increased, in three of cows with rumen acidosis it was greatly increased whereas in others did not developed at all. Rumen contents of both of the sick animal groups were considered were being hypoactivity.

67 Total protein, glucose, pyruvate, L-Lactate and TPP effect values in healty animals were found to be 7.25 g/dl, 55.05 mg/dl, 0.17 mg/dl, 10.82 mg/dl and 18.95% respectively, whereas in cows with hypoactivity in rumen microflora and fauna, these values were 6.74 g/dl, 52.5 mg/dl, 0.26 mg/dl, 11.9 mg/dl and 38.2%; and in cows with rumen acidosis were 8.04 g/dl, 70.8 mg/dl, 0.4 mg/dl, 19.5 mg/dl and 46.65% respectively. Total protein value in cows with hypoactivity in rumen microflora and fauna was decreased (p<0.001) whereas in cows with rumen acidosis increased (p<0.001) compared to healty animals. Glucose and L-Lactate values in cows with rumen acidosis were increased significantly (p<0.001) compared to both healty cows and cows with hypoactivity in rumen microflora and fauna. Pyruvate value in both cows with hypoactivity in rumen microflora and fauna and with rumen acidosis were increased significantly (p<0.001) compared to healty animals. In addition pyruvate value in cows with rumen acidosis were increased significantly (p<0.001) compared to cows with hypoactivity in rumen microflora and fauna. Values of TPP effect in both cows with rumen acidosis and with hypoactivity in microflora and fauna were found to be increased significantly (p<0.001) compared to control group. In conclusion, as it can be seen from pyruvate, L-Lactate and especially TPP effect values, slight thiamin deficiency in cows with hypoactivity in rumen microflora and fauna and prominent thiamin deficiency in cows with rumen acidosis are present.Therefore, we believe that the administration of thiamin preparates especially in cows with rumen acidosis would be useful.66 pulse rate in cows with rumen acidosis were significantly greater (p<0.001) than the other groups. Number of rumen contractions in two groups of sick animals was decreased compared to healty animals (p<0.001). Furthermore number of rumen motility in cows with rumen acidosis were found to be decreased significantly (p<0.001) as compared to that in cows with hypoactivity in rumen microflora and fauna. Avarage sedimentation rate in cows with hypoactivity in rumen microflora and fauna was decreased compared to that healty animals, whereas the sedimentation rate in cows with rumen acidosis was increased. Avarage flotation rate was found to be increased in cows with hypoactivity in rumen microflora and fauna compared to healty animals whereas in cows with rumen acidosis, no flotation was detected. Mean pH value was increased in cows with hypoactivity in rumen microflora and fauna whereas decreased in cows with rumen acidosis as compared to healty animals. Intensity of infusoria was found to be (+++) in healty animals whereas in cows with hypoactivity in rumen microflora and fauna was decreased (+) and in cows with rumen acidosis no infusoria was detectable (-) with the exception of three animals. Rate of living infusoria was decreased in cows with hypoactivity in microflora and fauna, whereas in cows with rumen acidosis, it was found that all the infusoria were dead. Period of methylen blue test in cows with hypoactivity in microflora and fauna was increased, in three of cows with rumen acidosis it was greatly increased whereas in others did not developed at all. Rumen contents of both of the sick animal groups were considered were being hypoactivity.67 Total protein, glucose, pyruvate, L-Lactate and TPP effect values in healty animals were found to be 7.25 g/dl, 55.05 mg/dl, 0.17 mg/dl, 10.82 mg/dl and 18.95% respectively, whereas in cows with hypoactivity in rumen microflora and fauna, these values were 6.74 g/dl, 52.5 mg/dl, 0.26 mg/dl, 11.9 mg/dl and 38.2%; and in cows with rumen acidosis were 8.04 g/dl, 70.8 mg/dl, 0.4 mg/dl, 19.5 mg/dl and 46.65% respectively. Total protein value in cows with hypoactivity in rumen microflora and fauna was decreased (p<0.001) whereas in cows with rumen acidosis increased (p<0.001) compared to healty animals. Glucose and L-Lactate values in cows with rumen acidosis were increased significantly (p<0.001) compared to both healty cows and cows with hypoactivity in rumen microflora and fauna. Pyruvate value in both cows with hypoactivity in rumen microflora and fauna and with rumen acidosis were increased significantly (p<0.001) compared to healty animals. In addition pyruvate value in cows with rumen acidosis were increased significantly (p<0.001) compared to cows with hypoactivity in rumen microflora and fauna. Values of TPP effect in both cows with rumen acidosis and with hypoactivity in microflora and fauna were found to be increased significantly (p<0.001) compared to control group. In conclusion, as it can be seen from pyruvate, L-Lactate and especially TPP effect values, slight thiamin deficiency in cows with hypoactivity in rumen microflora and fauna and prominent thiamin deficiency in cows with rumen acidosis are present.Therefore, we believe that the administration of thiamin preparates especially in cows with rumen acidosis would be useful.