Mentha pulegium L. ve Salvia tomentosa miller bitkilerinin metanol özütlerinin in vitro antioksidan aktivitelerinin belirlenmesi

Abstract

Mentha pulegium L. ve Salvia tomentosa Miller B T İ E İ İ ME A OİK L R N N TNLÖ Ü L R N Nin vitro A T O SD NA T V T L R N NB L R E ME İZ T E İİ N İ K İ A K İ İE E İ İ E İ L N SF ra E Y Ğ Teyt R İ İKm a nb i D l Y ke Lsn T z 68 s, 2006i y A aim a, üsk i s eil ı a ,Anahtar Kelimeler: Antioksidan aktivite, DPPH, Mentha pulegium L. , Salviatomentosa MillerB çlm d,Mentha pulegium L. ve Salvia tomentosa Miller bitkileri metanolu aş aaıözütlerinin antioksidan aktiviteleri incelendi. Antioksidan aktivite, ï¢-karoten yöntemi,serbest radikal giderim yöntemi (DPPH), indirgeme gücüv t l f o kb eie o a e l işpm ni l km k r g i4 f k t tugl a k bll d H ri b k i öü e gçia itı b a l e yu nr er ni e k i i n ztr ülrı s aa ie . i tn li üantioksidan aktivite gösterdi. M. pulegium ve S. tomentosa bitkilerinin metanolözütlerinin 2 gL-1 dri l ii lkaie ii nnnl iperoksidasyonu üzerinee ş e l o i s m ly uu i tim r n e t so psaı a %7,83 ve %75,0 i i so gs riKys nı na an dri dkıs lr y; 1 0 n b yn öt d ı l d ı , yı e ş eihi e . aa ğ d imstandart antioksidan BHT, lineloik asit emilsiyonunun lipit peroksidasyonu üzerine%95,96 inhibisyon gösterdi. M. pulegium ve S. tomentosa bitkilerinin metanolöü e n t dri l i0 0,10, 0,20 ve 0,40 mg/L) güçlü bir indirgeme gücüneztr i ü e ş e (,04,l i n m im rv sretai l i r i sh t. uaivt öü e ni ri f o kb eie e s r k g e m n ai i B n l e n ztr ç d ie l işb d a d i e pr ae li e ğ n i l km k r r p oa klşee olarak belirlendi.i a a ,i kt o edğrtlı r e iJüri: Prof. DrMut a E G Z. s fC N İaDoç. Dr. Ahmet GÜLCEYrd. Doç. Dr. Fethiye GÖDE

IN VITRO DETERMINATION OF ANTIOXIDANT ACTIVITY OFMETHANOLIC EXTRACT OF Mentha pulegium L. and Salvia tomentosa MillerF ra E Y Ğ Teyt R İ İChemistry Department, Post Gradute Thesis, 68 pg, 2006Key Words: Antioxidant activity, DPPH, Mentha pulegium L. , Salvia tomentosa MillerIn this study, the antioxidant activity of methanol extracts of Mentha pulegium L. andSalvia tomentosa (Miller) was studied. The antioxidant activity was investigated withfu d f et e ost βor ie n m t d:h -carotene bleaching test, the 2,2-diphenyl-1-picrylhydrazylfr h e ?(DPPH) radical scavenging method, reducing power and total phenolic. The extracts oftwo plants exhibited strong total antioxidant activity. At the concentrations of 2 gL-1,methanolic extracts of M. pulegium and S. tomentosa showed 71.83% and 75.00%inhibition on lipid peroxidation of linoleic acid emulsion, respectively. Comparable, 2gL-1 of standard antioxidant, butylated hydroxytoluene (BHT), exhibited 95.96%inhibition on peroxidation of linoleic acid emulsion. The extracts of M. pulegium and S.tomentosa had effective reductive potential and free radical scavenging at all testedamounts (0.04, 0.10, 0.2 and 0.4 mg/L). In addition, total phenolic compounds in the theextracts of two plants were determined as pyrocatechol equivalent.Jr: Po. rMut a E G Zuy rfD . s fC N İ aDoç. Dr. Ahmet GÜLCEYrd. Doç. Dr. Fethiye GÖDE