Koç spermasının yoğun gliserollü sulandırıcılarla dondurulması ve eritme sonrası fertilite üzerine etkileri

Abstract

83 observed to be high in high glycerol containing extenders (B2, B3l C2, C3) relating to the others(P<0.05). At the end of the first stage, it is concluded that, re-dilution techniques of ram semen without any change in the glycerol levels after equilibration have some harmful effects on motility at 5°C, this harmful effect was inhibited by high glycerol levels but high glycerol levels have deleterious effect on morphology specially on the acrosome. Second stage: in this stage, Bi and Ci were left out of the study because scored bad results. Same operations were done to the same groups(B0, B2, B3, C0, C2, C3) until the end of equilibration like in the first stage(n=20). At the end of equilibration and re-dilution semen was frozen immediately in pellets and stored in liquid-nitrogen. The best post-thaw motility records were 43.97±14.50% in group B0 and 37.72±17.83% in group C2. The difference between these groups was not significant(n=200). Motility was lower in the other groups(B2, B3, C0, C3; P<0.05). When the B(B0, B2, B3) and C(C0, C2, C3) groups were compared among themselves, it was observed that acrosome and total morphological defects increased with the increase in glycerol level. At the 2nd stage, it was decided that B0 (control) group have the quality to be used for freezing ram semen and artificial inseminations studies. In the 3rd stage semen was collected by electro-ejaculator in the breeding season for insemination studies(n=3). 5% glycerol containing extender(Group B0) was frozen according to the technique explained in the 2nd stage. Vaginal sponges containing 40 mg FGA were administered to 50 ewes. 500 I.U. PMSG was administered at the withdrawal day of the sponges. 45 ewes on heat were divided in to two groups 24(1. group) and 21(2.group) ewes respectively The first group(treatment) were inseminated with semen frozen in pellets. For the inseminations of the second group(control), semen was collected by electro- ejaculator and diluted with extender A and 0.2 ml insemination volumes were used. 100x106/ml doses were used in the both groups. On the 18th day after inseminations, serum progesterone was determined by RIA technique and ewes having progesterone levels higher then 1ng./ml were considered pregnant. Pregnancy rates were 50.0% in the first group and 61.9% in84 the second group and the difference between these groups was not significant. Duncan tests were used for the statistical evaluations of spermatological findings and t-tests for pregnancy rates. At the end, 5% glycerol addition to the skimmed milk powder-fructose-sodium citrate- a-tocopherol acetate- egg yolk containing ram semen extender(modified extender A) was observed to have beneficial effects on post-thaw spermatological characters, fertility results and practical use.83 observed to be high in high glycerol containing extenders (B2, B3l C2, C3) relating to the others(P<0.05). At the end of the first stage, it is concluded that, re-dilution techniques of ram semen without any change in the glycerol levels after equilibration have some harmful effects on motility at 5°C, this harmful effect was inhibited by high glycerol levels but high glycerol levels have deleterious effect on morphology specially on the acrosome. Second stage: in this stage, Bi and Ci were left out of the study because scored bad results. Same operations were done to the same groups(B0, B2, B3, C0, C2, C3) until the end of equilibration like in the first stage(n=20). At the end of equilibration and re-dilution semen was frozen immediately in pellets and stored in liquid-nitrogen. The best post-thaw motility records were 43.97±14.50% in group B0 and 37.72±17.83% in group C2. The difference between these groups was not significant(n=200). Motility was lower in the other groups(B2, B3, C0, C3; P<0.05). When the B(B0, B2, B3) and C(C0, C2, C3) groups were compared among themselves, it was observed that acrosome and total morphological defects increased with the increase in glycerol level. At the 2nd stage, it was decided that B0 (control) group have the quality to be used for freezing ram semen and artificial inseminations studies. In the 3rd stage semen was collected by electro-ejaculator in the breeding season for insemination studies(n=3). 5% glycerol containing extender(Group B0) was frozen according to the technique explained in the 2nd stage. Vaginal sponges containing 40 mg FGA were administered to 50 ewes. 500 I.U. PMSG was administered at the withdrawal day of the sponges. 45 ewes on heat were divided in to two groups 24(1. group) and 21(2.group) ewes respectively The first group(treatment) were inseminated with semen frozen in pellets. For the inseminations of the second group(control), semen was collected by electro- ejaculator and diluted with extender A and 0.2 ml insemination volumes were used. 100x106/ml doses were used in the both groups. On the 18th day after inseminations, serum progesterone was determined by RIA technique and ewes having progesterone levels higher then 1ng./ml were considered pregnant. Pregnancy rates were 50.0% in the first group and 61.9% in

84 the second group and the difference between these groups was not significant. Duncan tests were used for the statistical evaluations of spermatological findings and t-tests for pregnancy rates. At the end, 5% glycerol addition to the skimmed milk powder-fructose-sodium citrate- a-tocopherol acetate- egg yolk containing ram semen extender(modified extender A) was observed to have beneficial effects on post-thaw spermatological characters, fertility results and practical use.83 observed to be high in high glycerol containing extenders (B2, B3l C2, C3) relating to the others(P<0.05). At the end of the first stage, it is concluded that, re-dilution techniques of ram semen without any change in the glycerol levels after equilibration have some harmful effects on motility at 5°C, this harmful effect was inhibited by high glycerol levels but high glycerol levels have deleterious effect on morphology specially on the acrosome. Second stage: in this stage, Bi and Ci were left out of the study because scored bad results. Same operations were done to the same groups(B0, B2, B3, C0, C2, C3) until the end of equilibration like in the first stage(n=20). At the end of equilibration and re-dilution semen was frozen immediately in pellets and stored in liquid-nitrogen. The best post-thaw motility records were 43.97±14.50% in group B0 and 37.72±17.83% in group C2. The difference between these groups was not significant(n=200). Motility was lower in the other groups(B2, B3, C0, C3; P<0.05). When the B(B0, B2, B3) and C(C0, C2, C3) groups were compared among themselves, it was observed that acrosome and total morphological defects increased with the increase in glycerol level. At the 2nd stage, it was decided that B0 (control) group have the quality to be used for freezing ram semen and artificial inseminations studies. In the 3rd stage semen was collected by electro-ejaculator in the breeding season for insemination studies(n=3). 5% glycerol containing extender(Group B0) was frozen according to the technique explained in the 2nd stage. Vaginal sponges containing 40 mg FGA were administered to 50 ewes. 500 I.U. PMSG was administered at the withdrawal day of the sponges. 45 ewes on heat were divided in to two groups 24(1. group) and 21(2.group) ewes respectively The first group(treatment) were inseminated with semen frozen in pellets. For the inseminations of the second group(control), semen was collected by electro- ejaculator and diluted with extender A and 0.2 ml insemination volumes were used. 100x106/ml doses were used in the both groups. On the 18th day after inseminations, serum progesterone was determined by RIA technique and ewes having progesterone levels higher then 1ng./ml were considered pregnant. Pregnancy rates were 50.0% in the first group and 61.9% in84 the second group and the difference between these groups was not significant. Duncan tests were used for the statistical evaluations of spermatological findings and t-tests for pregnancy rates. At the end, 5% glycerol addition to the skimmed milk powder-fructose-sodium citrate- a-tocopherol acetate- egg yolk containing ram semen extender(modified extender A) was observed to have beneficial effects on post-thaw spermatological characters, fertility results and practical use.