Ratlarda safra kanalı bağlanması ile oluşan kolestatik karaciğer hasarına karşı cape`nin koruyucu etkisi

Abstract

ÖZET Bilier obstrüksiyon yapılan ratlarda CAPE'nin karaciğer üzerine koruyucu etkisini araştırdık. Bilier obstrüksiyon 14 gün devam etti. Ratlar 3 gruba ayırıldı. Grup A; BO Grup B; BO+CAPE Grupo C; Kontrol. GGT, AST, ALT seviyeleri CAPE verilen grupta azaldı. MDA, GSH, MPO seviyeleri anti-oksidan düzeyleri için çalışıldı. Sonuçta CAPE'nin BO sonrası oluşan karaciğer hasarına karşı koruyucu etkisi olduğu görüldü. 33

SUMMARY Objectives: We investigated the protective role of caffeic acid phenethyl ester (CAPE) in rat liver injuries induced by chronic biliary obstruction. Methods: Secondary biliary cirrhosis was induced by bile duct ligation for 14 days. Swiss albino rats were divided into 3 groups: Common bile duct ligated (CBDL) rats; Group A, CBDL rats treated with CAPE as Group B and simple laparotomy group known as Sham group; Group C. Group B received 10-4,mol/kg dose of CAPB intraperitoneal^ daily throughout 14 days. Results: The present data showed a decreased Gama glutamyl transferase (GGT) levels; aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in the CAPE treated rats, when compared with CBDL rats (p<0.05). In the CAPE treated rats, tissue levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were significantly lower and the levels of glutathione (GSH) were significantly higher than that of CBDL rats (pO.OOl, pO.OOl respectively). The levels of Interleukin-la (IL-la) and Interleukin-6 (IL-6) in CAPE treated rats were significantly lower than that of CBDL rats (p<0.03, p<0.02 respectively). Administration of CAPE in the rats with biliary obstruction resulted in inhibition of necro-inflammation, shown histopathologically. Conclusion: The present study demonstrates that intraperitoneal administration of CAPE in CBDL rats maintains antioxidant defenses, reduces liver oxidative and 34cytokin damage and necro-inflammation. This effect of CAPE may be useful in the preservation of liver injury in cholestasis. 35